Optimization of Bifonazole-Loaded Nisomal Formulation Using Plackett-Burman Design and 23 Factorial Design
Harshal Ashok Pawar*, Vibhavari Bhaskar Attarde, Gide Parag Subhash
Identifiers and Pagination:Year: 2016
First Page: 31
Last Page: 48
Publisher Id: PHARMSCI-3-31
Article History:Received Date: 3/4/2015
Revision Received Date: 5/4/2016
Acceptance Date: 05/04/2016
Electronic publication date: 06/05/2016
Collection year: 2016
open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution-Non-Commercial 4.0 International Public License (CC BY-NC 4.0) (https://creativecommons.org/licenses/by-nc/4.0/legalcode), which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
Bifonazole is an imidazole antifungal drug. It is highly lipophilic drug with a very short half-life and is minimally absorbed following dermal application. Niosomes are excellent candidate as potential drug delivery system because of their improved drug solubilization, enhanced penetration power, long shelf life and ease of preparation and administration.
The aim of the study was to enhance permeability of bifonazole by incorporating it into niosomes and formulating its gel formulation.
Niosomes were prepared by thin film hydration method. The statistical approach was used to optimize the parameters for niosome formation. The process parameters among six factors influencing in vitro diffusion study were identified using plackett burman design. The Plackett–Burnan design described in this study was applied for the identification of the significant factors and 23 factorial design was used to obtain optimized batch. The prepared niosomes were evaluated for various parameters such as vesicular size, drug entrapment, surface morphology, drug release characteristics, ex vivo deposition and stability.
Niosomal gel showed significantly enhanced drug permeation through the skin as compared to the marketed formulation. The optimized niosomal gel formulation also showed drug release in a controlled manner. The stability study at two different temperatures (25 ± 2°C and and 5 ± 3 °C) confirmed that the optimized formulation is stable even at the end of 3 months.
It has been concluded that niosomal gel is an efficient carrier for the delivery of bifonazole with improved permeability.